The effects of in vitro culture on the leaf structure of rootstock CAB 6P (Prunus cerasus)

AVDIRRAHMAN GASHI¹*, KRISTAQ SINI ², EDLIRA KUKALI³

¹Faculty of Agricultural & Environment, Agricultural University of Tirana

²Faculty of Biotechnology, Agricultural University of Tirana

³Faculty of Agricultural & Environment, Agricultural University of Tirana

*Corresponding author e-mail: avdi-gashi773@hotmail.com

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Abstract

Stomatal apparatus are epidermal characters that are very important in characterizing the plant species and represent a large variability between in vivo plants growths, in vitro acclimatized.

In our study during 2018 year, epidermal characters are compared (stomata) of the in vivo P. Mahaleb (prunus cerasus L) and acclimatized to CAB 6P (prunus cerasus L) in vitro.

Epidermal characters which were observed were: distribution and stomatal type, stomatal number (stomatal density) for the whole microscopic field / for leaf surface units in the plantlets of two variants prunus cerasus rootstock CAB 6P studied during the acclimatization of in vitro cultivation and P. Mahaleb in vivo, the leaves are hystomatic with anomocytic stomatal apparatus.

Both variants of in vivo and in vitro cultivation, it turns out that stomata of the down epidermis have elliptical guard cells. Stomatal density is higher in “in vivo” plantlets compared to those of “in vitro” acclimated, due to high air humidity and increased intensity of lighting.

The values of the search indicators are presented with a variable for the respective variants in two rootstocks P. Mahaleb (in vivo) and CAB 6 P (in vitro), respectively: the number of accompanying cells was 5-7 to 5; stomatal length 18.2 to 16.7; stomatal width (μm) 11.3 with 10.4 and average stomatal density (mm2) 39 with 36.

Keywords: in vivo, in vitro, stomata, acclimatization, Prunus cerasus L