Optimization of explants sterilizations intended for In vitro culture of sp. Prunus avium

AVDIRRAHMAN GASHI, TOKLI THOMAJ , TAULANT MITRUSHI,  EDLIRA KUKALI

Faculty of Agricultural & Environment, Agricultural University of Tirana &Faculty of Agricultural & Environment, Agricultural University of Tirana,

In Vitro laboratory Elbasan.

*Corresponding author E-mail: avdi-gashi773@hotmail.com

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Abstract

The aim of research was establishment and maintenance of in vitro explants free from bacteria. PrunusAviumexplants intended for tissue culture as object of research treated with different concentrations of sterilizante. The presence of bacterial contaminants is extremely important to micro propagate plants.

Different concentration of HgCl₂ (0.01; 0.02 %) and NaOCl (0.25; 0.5%) for varying exposure time (15; 20 min).The highest contamination (80%) and least explants survival were identification when used 0.02% HgCl₂ or 90 % with 0.25%NaOCl.

Minimum death of explants was in 0.01% HgCl₂ and 0.5 % NaOCl disinfected for 15 min respectively 90%- 95%.Isolated buds, after sterilization with 0.01% HgCl2 for 15 min are characterized by higher survival rate, respectively  85% for P. mahaleb and 92% for P. avium (rootstocks: i – P. avium x P canascens (Gisela 6)

Keywords: explants; prunusavium; sterilization;in vitro; concentration.