Vilson Bozgo, Lorena Hysi, Anila Hoda
Department of Animal Production, Faculty of Agriculture, Agricultural University of Tirana, Albania
Email: ahoda@ubt.edu.al
Abstract
Carbonyl reductase is a monomeric, cytosolic enzyme that catalyzes the two-electron reduction of a wide range carbonyc compounds. We intend to make a silico analysis of CRB gene in different vertebrate species. The homology is analysed with NCBI BLASTp, a multiple alignment is carried out by Clustal Omega and phylogenetic tree is constructed by Mega 6. CRB protein is highly conserved in the considered species. No transmembrane regions or signal peptides were detected. Subcellular localization analysis revealed that human CRB1 was a cytoplasmatic protein (62.5%). Results showed an entire open reading frame of 887 bp encoding 295 aminoacids. This gene is expressed in different tissues, but is highly expressed in small intestine, liver and colon
Keywords: carbonyl reductase, expression, bioinformatic, in silicoclonning
