Allelic frequencies of MFW7 microsatellite loccus in carp (Cyprinus carpio) of two fish farming centers

Xhiliola Bixheku¹, Anila Hoda^2*, Dhurata Bozo¹

¹Public Acredition Agency for Higher Education

²Agricultural University of Tirana, Albania

Coresponding author email: ahoda@ubt.edu.al

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Abstract

Common carp (Cyprinus carpio L) is among the most important freshwater fish species which are distributed all over the world. We present preliminary data on genotyping individuals by microsatellite marker. Allelic frequencies of one microsatellite loci was estimated on 60 individuals sampled randomly on Tapiza and Belsh fish farming centers. Fragemts were aplified by Polymerase Chain Reactions (PCR), and afterwards were separated on a 6% PAA denaturing gel. A total of 23 alleles were detected. Mean observed heterozygosity was 0.334 and expected heterozygosity was 0.816.

Keywords: Observed and expected heterozygosity, marker